苏州市衰老与神经疾病重点实验室

秦正红教授课题组

秦正红博士，教授

1994获美国宾州医学院药理学博士学位。1994-1999年在美国国家卫生研究院(NIH)从事博士后工作，1999-2002年在哈佛大学任讲师。2003年引进苏州大学，现为苏州大学药理学特聘教授，博导，苏州市衰老与神经疾病重点实验室主任。

秦正红教授长期从事神经元死亡机制研究，主要开展神经退行性疾病及缺血性脑卒中的基础和实验治疗研究，已经取得了较大成绩。以苏州大学为署名单位发表的SCI论文已有50余篇。先后主持国家自然科学基金面上项目4项及重点项目1项，江苏省自然科学基金项目1项，教育部博士点专项基金项目1项，江苏省六大高峰人才项目1项，国家科技部863重大专项1项，作为骨干参加国家科技部973项目1项。已申请发明专利8项，已获得发明专利4项。参编、主编教材和专著7部。

目前承担及参与的科研情况

1.   科技部973项目(2011CB510003)：自噬对多巴胺神经元损伤的调控，2011-2016，247万。

2.  科技部863项目(2008AA02Z436)：高保真聚合酶介导的突变敏感性分子开关在重大疾病诊断中的应用，2008-2011，273万。

3.  国家自然科学基金重点项目(30930035)：自噬与凋亡相互作用的分子机制及其在神经细胞生存中的意义，2010-2013，170万。

近五年的代表性文章

•Rui Sheng, Li-Sha Zhang, Rong Han, Xiao-Qian Liu, Bo Gao and Zheng-Hong Qin. Autophagy activation is associated with neuroprotection in a rat model of focal cerebral ischemic preconditioning. Autophagy, 2010, 6(4): 482-494. (Corresponding author)

•Ai-Ping Qin, Chun-Feng Liu, Yuan-Yuan Qin, Li-Zhi Hong, Min Xu, Lei Yang, Jianqing Liu, Zheng-Hong Qin and Hui-Ling Zhang. Autophagy was activated in injured astrocytes and mildly decreased cell survival following glucose and oxygen deprivation and focal cerebral ischemia. Autophagy, 6(6): 738-753. (Co-Corresponding author)

•Yan Wang, Zheng-hong Qin. Molecular and cellular mechanisms of excitotoxic neuronal death. Apoptosis, 2010, 15: 1382-1402. (Corresponding author)

•Yan Wang, Xiao-Xia Dong, Yi Cao, Zhong-Qin Liang, Rong Han, Jun-Chao Wu, Zhen-Lun Gu and Zhen-Hong Qin. p53 induction contributes to excitotoxic neuronal death inrat striatum through apoptotic and autophagic mechanisms. European Journal of Neuroscience, 2009, 30: 2258-2270. (Corresponding author)

•Xing-Ding Zhang, Ye Wang, Yan Wang, Xuan Zhang, Rong Han, Jun-Chao Wu, Zhong-Qin Liang, Zhen-Lun Gu, Feng Han, Kohji Fukunaga and Zheng-Hong Qin. p53 mediates mitochondria dysfunction-triggered autophagy activation and cell death in rat striatum. Autophagy, 2009, 5(3): 339-350. (Corresponding author)

•Xing-Ding Zhang, Ye Wang, Jun-Chao Wu, Fang Lin, Rong Han, Feng Han, Kohji Fukunaga, and Zheng-Hong Qin. Down-Regulation of Bcl-2 Enhances Autophagy Activation and Cell Death Induced by Mitochondrial Dysfunction in Rat Striatum. Journal of Neuroscience Research, 2009, 87: 3600-3610. (Corresponding author)

•Yan Wang, Rong Han, Zhong-Qin Liang, Jun-Chao Wu, Xing-Ding Zhang, Zhen-Lun Gu and Zheng-Hong Qin. An autophaic mechanism is involved in apoptotic death of rat striatal neurons induced by the non-N-methyl-D-aspartate receptor agonist kainic acid. Autophagy, 2008, 4(2): 214-226. (Corresponding author)

•Hui-Ling Zhang, Zhen-Lun Gu, Sean I. Savitz, Feng Han, Kohji Fukunaga, and Zheng-Hong Qin. Neuroprotective effects of prostaglandin A1 in rat models of permanent focal cerebral ischemia are associated with nuclear factor-κB inhibiton and peroxisome proliferator-activated receptor-γup-regulation. J Neurosci Res, 2008, 86: 1132-1141. (Corresponding author)

•Ya-Dan Wen, Rui Sheng, Li-Sha Zhang, Rong Han, Xuan Zhang, Xing-Ding Zhang, Feng Han, Kohji Fukunaga and Zheng-Hong Qin. Neuronal injury in rat model of permanent focal cerebral ischemia is associated with activation of autophagic and lysosomal pathways. Autophagy, 2008, 4(6): 762-769. (Corresponding author)

•Zhong-Qin Liang, Xiao-Xia Wang, Yu-Mei Wang, Ren-Wu Chen, De-Maw Chuang, Thomas N. Chase, Zheng-Hong Qin. Nuclear factor-κB-dependent clyclin D1 induction and DNA replication associated with N-methyl-D-aspartate receptor mediated apoptosis in rat striatum. J Neurosci Res, 2007, 85: 1295-1309. (Corresponding author)

•Zhong-Qin Liang, Yun-Lin Li, Xi-Lin Zhao, Rong Han, Xiao-Xia Wang, Yu-Mei Wang, Thomas N. Chase, M. Catherine Bennett, Zheng-Hong Qin. NF-κB contributes to 6-hydroxydopamine-induced apoptosis of nigral dopaminergic neurons through p53. Brain Res, 2007, 45: 190-203. (Corresponding author)

•Hui-Ling Zhang, Rong Han, Zhi-Xin Chen, Bo-Wen Chen, Zhen-Lun Gu, P F Reid, L N Raymond, Zheng-Hong Qin. Opiate and acetylcholine-independent analgesic actions of Crotoxin. Toxicon, 2006, 48: 175-182. (Corresponding author)

•Ci-Hui Yan, Zhong-Qin Liang, Zhen-Lun Gu, Ya-Ping Yang, P F Reid, Zheng-Hong Qin. Contributions of autophagic and apoptotic mechanisms to crotoxin-induced death of K562 cells. Toxicon, 2006, 47: 521-530. (Corresponding author)

•Xi-Hui Xu, Hui-Ling Zhang, Rong Han, Zhen-Lun Gu, Zheng-Hong Qin. Enhancement of neuroprotection and heat shock protein induction by combined prostaglandin A1 and lithium in rodent models of focal ischemia. Brain Res, 2006, 1102: 154-162. (Corresponding author)

•Yan Wang, Zhen-Lun Gu, Yi Cao, Zhong-Qin Liang, Rong Han, M C Bennett, Zheng-Hong Qin. Lysosomal enzyme cathepsin B is invovled in kainic acid-induced excitotoxicity in rat striatum. Brain Res, 2006, 1071: 245-249. (Corresponding author)

主要研究方向

1. 神经退行性疾病致病分子机制的研究：以神经兴奋性毒素、化学药物毒素、致病突变基因表达制作帕金森病、亨廷顿舞蹈病等的动物及细胞模型，研究自噬/溶酶体机制在疾病的致病分子机制中发挥的重要作用。

2. 缺血性脑卒中及缺血预适应分子机制研究：利用大、小鼠中动脉缺血模型研究自噬/溶酶体途径、内质网应激在缺血性脑卒中及缺血预适应中的分子作用机制。

3. 生物毒素药用开发：对中华眼镜蛇毒进行毒理药理研究，经物理改性使蛇毒减毒增效，开发蛇毒活性成分在镇痛和治疗自身免疫性疾病治疗中的药用价值并开发成临床治疗的不同剂型的药物。

秦正红课题组成员

林芳：副教授，研究方向为自噬/溶酶体途径与内质网应激在亨廷顿舞蹈病突变蛋白清除中的作用机制。

代表文章：

1. Lin F, Wu JC, Wang Y, Qin ZH. Huntingtin Cleavage by Thrombin In vitro. Acta Biochimica et Biophysica Sin. 2007, 39(1): 15–18.

2. Lin F, Xu SL, Ni WM, Chu ZQ, Xu ZH, Xue HW. Identification of ABA-responsive genes in rice shoots via cDNA macroarray. Cell Res. 2003, 13: 59-68.

3. Wang LH, Lin F, Wu JC, Qin ZH. High Efficiency Adenovirus-Mediated Expression of Truncated N-terminal Huntingtin Fragment (htt552) in Primary Rat Astrocytes. Acta Biochim Biophys Sin, 2009, 41: 325-334.

盛瑞：副教授，研究方向为自噬和内质网应激在缺血性脑卒中及缺血预适应中的作用机制。目前作为主持人负责两项国家自然科学基金：自噬/溶酶体途径激活在缺血性脑中风星形胶质细胞死亡中的作用及机制(30973510)；内质网应激诱导自噬在缺血预适应和致死性缺血中的不同作用(81173057)。

代表文章：

1. Rui Sheng, Li-Sha Zhang, Rong Han, Xiao-Qian Liu, Bo Gao and Zheng-Hong Qin. Autophagy activation is associated with neuroprotection in a rat model of focal cerebral ischemic preconditioning. Autophagy, 2010, 6(4): 482-494.

2. Rui SHENG, Li-sha ZHANG, Rong HAN, Bo GAO, Xiao-qian LIU, Zheng-hong QIN. Combined prostaglandin E1 and lithium induces Bcl-2 and heat shock proteins while inhibiting p53 in a rat model of cerebral ischemia. Acta Pharmacologica Sinica, 2011, 1: 1-8.

3. Sheng R, Wen YD, Zhang LS, Han R, Zhang X, Zhang XD, Han F, Kohji F, Qin ZH. Neuronal injury in rat model of permanent focal cerebral ischemia is associated with activation of autophagic and lysosomal pathways. Autophagy 2008; 4(6): 762-9.

蒋小岗：副教授，研究方向为亨廷顿舞蹈病蛋白在调节自噬中的作用。目前作为主持人负责一项国家自然科学基金，应用SILAC技术研究亨廷顿蛋白在调节自噬中的作用(81102430)。

代表文章：

1. Jiang XG, Dong J, Wang F, Feng S, Ye M Zou H. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column. Electrophoresis. 2008, 29, 1612-1618.

2. Jiang XG, Ye ML, Zou HF. Technologies and methods for sample pretreatment in efficient proteome and peptidome analysis. Proteomics. 2008, 8: 686-705.

3. Jiang XG, Feng S, Tian R, Han G, Jiang XN Ye ML, Zou HF. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column. Proteomics. 2007, 7: 528-539.

王燕：讲师，研究方向为p53及NF-kB信号通路在兴奋性毒素引起的神经元死亡中的作用。目前作为主持人负责一项国家自然科学基金，神经兴奋性毒性中Cathepsin L降解IκB-α的机制及意义(81000547)。

1. Yan Wang, Zheng-hong Qin. Molecular and cellular mechanisms of excitotoxic neuronal death. Apoptosis, 2010, 15: 1382-1402.

2. Yan Wang, Xiao-Xia Dong, Yi Cao, Zhong-Qin Liang, Rong Han, Jun-Chao Wu, Zhen-Lun Gu and Zhen-Hong Qin. p53 induction contributes to excitotoxic neuronal death inrat striatum through apoptotic and autophagic mechanisms. European Journal of Neuroscience, 2009, 30: 2258-2270.

3. Wang Y, Han R, Liang QZ, Wu JC, Zhang XD, Gu ZL, Qin ZH. An autophagic mechanism is involved in apoptotic death of rat striatal neurons induced by the non-N-methyl-D-aspartate receptor agonist kainic acid. Autophagy. 2008, 4(2): 214-226.

韩蓉：高级实验师，苏州市衰老与神经疾病重点实验室行政秘书。研究方向为神经毒素的毒理药理研究及药用开发。

代表文章：

1. Han R, Gao B, Sheng R, Zhang LS, Zhang HL, Gu ZL, Qin ZH. Synergistic effects of prostaglandin E1 and lithium in a rat model of cerebral ischemia. Acta Pharmacologica Sinica 2008, 29:1141-1149.

2. Wang Y, Han R, Liang QZ, Wu JC, Zhang XD, Gu ZL, Qin ZH. An autophagic mechanism is involved in apoptotic death of rat striatal neurons induced by the non-N-methyl-D-aspartate receptor agonist kainic acid. Autophagy. 2008, 4(2): 214-226.

3. Zhang HL, Han R, Chen ZX, Chen BW, Gu ZL, Reid PF, Raymond LN, Qin ZH. Opiate and Acetylcholine-independent Analgesic Actions of Crotoxin. Toxicon  2006, 48:175-182.

邬珺超：助理研究员，苏州市衰老与神经疾病重点实验室科研秘书。研究方向为自噬/溶酶体途径参与环境因素及遗传因素所致神经退行性疾病的分子机制。

代表文章：

1. Wu JC, Liang ZQ, Qin ZH. Quality Control System of the Endoplasmic Reticulum and Related Diseases. Acta Biochem Biophys Sin. 2006, 38: 219-226.

2. Wu JC, Lin F, Qin ZH. Sequestration of GAPDH to Aggregates Formed by Mutant Huntingtin. Acta Biochem Biophys Sin. 2007, 39: 885-890.

3. Lin F, Wu JC, Wang Y, Qin ZH. Huntingtin Cleavage Induced by Thrombin in Vitro. Acta Biochem Biophys Sin. 2007, 39: 15-18.